Mutation-Specific RAS Oncogenicity Explains NRAS Codon 61 Selection in Melanoma
نویسندگان
چکیده
منابع مشابه
Mutation-specific RAS oncogenicity explains NRAS codon 61 selection in melanoma.
UNLABELLED NRAS mutation at codons 12, 13, or 61 is associated with transformation; yet, in melanoma, such alterations are nearly exclusive to codon 61. Here, we compared the melanoma susceptibility of an NrasQ61R knock-in allele to similarly designed KrasG12D and NrasG12D alleles. With concomitant p16INK4a inactivation, KrasG12D or NrasQ61R expression efficiently promoted melanoma in vivo, whe...
متن کاملNRAS Codon 61 Selection in Melanoma
showed enhanced nucleotide binding, decreased intrinsic GTPase activity, and increased stability when compared with Nras G12D . This work identifi es a faithful model of human NRAS -mutant melanoma, and suggests that the increased melanomagenecity of Nras Q61R over Nras G12D is due to heightened abundance of the active, GTP-bound form rather than differences in the engagement of downstream effe...
متن کاملDetection of a mouse H-ras codon 61 mutation using a modified allele-specific competitive blocker PCR genotypic selection method.
A modified allele-specific competitive blocker PCR (ACB-PCR) has been developed as an approach for genotypic selection, the detection of a rare mutant allele based solely upon its altered nucleotide sequence. ACB-PCR genotypic selection operates through the preferential PCR amplification of mutant DNA using a primer that has more mismatches to the wild-type allele than the mutant allele. In add...
متن کاملHigh rates of ras codon 61 mutation in thyroid tumors in an iodide-deficient area.
Using polymerase chain reaction and sequence-specific oligonucleotide hybridization, the frequency of three ras oncogene mutations (N-ras, Ha-ras, and K-ras) in thyroid tumors (25 adenomas, 16 follicular carcinomas, and 22 papillary carcinomas) was investigated in both iodide-deficient and iodide-sufficient areas. The ras oncogene mutation rate was significantly higher in the iodide-deficient a...
متن کاملNRAS and BRAF mutation frequency in primary oral mucosal melanoma.
Oral mucosal melanoma (OMM) is a fatal sarcoma of unknown etiology. Histological morphology and genetic events are distinct from those of its cutaneous counterpart. Mutation and up-regulation of c-kit has been identified in OMM which may activate downstream molecules such as RAS and RAF. These molecules are involved in the mitogen-activated protein kinase (MAPK) pathway leading to tremendous ce...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cancer Discovery
سال: 2014
ISSN: 2159-8274,2159-8290
DOI: 10.1158/2159-8290.cd-14-0729